Experimental Plan

Over the course of our 5-day practical series we will be covering most of the protocol for lab scale fermentations from preparation of the seed culture all the way to harvesting, isolating and purifying the product of interest.

The flowchart below shows our experimental plan and what was done over the 5 days of practicals.

However, to aid the flow of information and for the sake of being viewer-friendly, some of the posts may include 2 or 3 days of experimental work in them. In such cases, the day number will be indicated to make it clear on exactly which day that part of the experiment was carried out.

On whole, this experiment aims to culture genetically-engineered Escherichia coli containing a foreign gene that codes for a protein called Green Fluorescent Protein (GFP). In addition, to also harvest these bacteria cells after fermentation and to isolate and purify it.

For further information on what GFP is and how genetic engineering of Escherichia coli (E. coli) is done, please refer to their respective posts.